By extension, we also evaluate the strategy of sensitizing GBM cells (using delivered antagomiRs) prior to TMZ treatment, and study the effects of both antagomiRs and TMZ on cellular proliferation, apoptosis, and inhibition of endogenous miRNAs function and their downstream effects on tumor suppressor and apoptotic gene (PTEN, PDCD4, HOXD10, P53 and CASP3) expression. This evidence concerns the gene PTEN and glioblastoma.