NAF1 and familial pancreatic carcinoma: To explore the role of NAF-1 in pancreatic cancer and its relationship with Nrf2, we first examined the expression levels of NAF-1 and Nrf2 in four different pancreatic cancer cell lines (Panc-1, Mia paca-2, BxPC-3, and CF pac-1) by Western blotting, real-time PCR, and immunofluorescence (Figures 2(a)–2(e)).