We found that Gal-9 expression was significantly associated with the depth of invasion (TI/TII vs. TIII/TIV, OR = 2.80, 95% CI = 1.97–3.96, P < 0.001), histopathological stage (I/II vs. III/IV, OR = 3.00, 95% CI = 2.04–4.42, P < 0.001), metastasis of lymph node (Presence vs. Absence, OR = 0.47, 95% CI = 0.25–0.89, P = 0.020) and distal tumor metastasis (Presence vs. Absence, OR = 13.85, 95% CI = 3.50–54.76, P < 0.001) but not with sex, vascular invasion or tumor number (Table 3). This evidence concerns the gene LGALS9 and neoplasm.