CD274 and breast cancer: To identify the N-glycosyltransferase(s) responsible for PD-L1 N-glycosylation and characterize the role of PD-L1 N-glycosylation in PD-L1 induction upon EMT, we scored the EMT status (a higher score indicates a more mesenchymal-like signature) of samples from The Cancer Genome Atlas (TCGA) breast cancer dataset (n = 1100) (Supplementary Data 1)31 and analyzed the correlations between N-glycosyltransferases and PD-L1 (CD274) mRNA expression, and EMT scores.