By analyzing these HCC tissue specimens, we found that, as shown in Fig. 6a, b, 85.7% and 64.3% of the samples with low EYA4 expression exhibited high levels of p-Ser32-IκB and RAP1, whereas 60.0% and 76.0% of the samples with high EYA4 expression showed low expression of p-Ser32-IκB and RAP1, respectively. Here, RAP1A is linked to hepatocellular carcinoma.