Intriguingly, the expression of EYA4 was inversely correlated with the p-Ser32-IκB and RAP1 levels in human HCC specimens, in agreement with our postulation that EYA4 deficiency is a major cause of the hyperactivated state of the NF-κB/RAP1 signaling axis in HCC. This evidence concerns the gene RAP1A and hepatocellular carcinoma.