Two highly suppressed TSGs in tissue samples, CHFR and RIZ1, were significantly suppressed in nasopharyngeal brushing samples obtained from NPC patients, whereas CACNA2D3, which was significantly down-regulated in tissues, did not differ significantly between NPC patients and healthy controls in nasopharyngeal brushing samples. This evidence concerns the gene CACNA2D3 and nasopharyngeal carcinoma.