Modeling of the association between TUBB6 methylation levels and presence of invasive disease (taken as either “field” mucosa, dysplasia, or cancer) using logistic regression found that a threshold of 17% methylation was sufficient to discriminate invasive disease correcting for a population prevelance of UC-associated dysplasia of 3% (AUC 0.84, 95% CI 0.81–0.87), Supplementary figure 2. This evidence concerns the gene TUBB6 and cancer.