To clarify the role of TLR2 and TLR9 in inflamed tissues after mucosal HSV infection, we assessed the survival of WT, TLR2 KO, TLR9 KO, and TLR2/9 DKO mice following vaginal infection with different doses of the HSV-1 McKrae strain (1 × 106, 1 × 107, and 5 × 107 pfu/mouse). This evidence concerns the gene TLR9 and infective vaginitis.