The study of ANKK1 proteins during myogenic differentiation of human rhabdomyosarcoma (RD) cell line also shows a decrease in the nuclear signal (D0-D3, t: 6.137, p <0.0001; D0-D6, t: 8.620, p <0.0001; D3-D6, t: 2.591, p = 0.0179) (Fig 2D–2F). This evidence concerns the gene ANKK1 and rhabdomyosarcoma.