CD40LG and Flavivirus Infections: For the purpose of validation of the assay for ZIKV diagnosis, 38 acute phase sera and 25 convalescent phase (collected > 7 days after the first) sera from patients positive for ZIKV (RT-PCR or PRNT confirmed cases) but who have no serological evidence of previous flavivirus infection (primary ZIKV cases), 834 acute and convalescent-phase sera known to be positive for one of nine other flaviviruses and 70 flavivirus IgG and IgM screening ELISA negative sera were analyzed by flaviMIA (Table S1).