AKT1 and Miyoshi myopathy: Supplementary Figure 1 shows that in MM-ECs treated with siRICTOR (25 nM for 72h) the RICTOR expression was strikingly reduced (-75% at both mRNA and protein levels). Immunoblotting analysis also revealed a significant reduction of both p-AKT and p-mTOR proteins in MM-ECs silenced for 72h (Supplementary Figure 1C).