This study produces significant results in four aspects: 1) c.35 G>A (G12D), c.35 G>T (G12V) and c.34 G>A (G12S) were detected in TNM stage II to IV CD166-pcc CRC specimens, 2) c.35 G>A (G12D), c.35 G>T (G12V), c.38G>A (G13D) and c.34G>A (G12S) were also detected in CAD, 3) a mutation c.34G>A (G12S) was found in both CRC and CAD specimens from the same patient and 4) prognostic significance of CD166-pcc KRAS exon 2 mutations in predicting poor treatment outcome. This evidence concerns the gene ALCAM and colorectal carcinoma.