The MDA-MB-231 tumor and neo-vessels in the tumor region could be clearly visualized after 3 min post injection. Exposure to the focused ultrasound triggered the collapse of the Porphyrin-MB-PTX-HSA NPs, resulting in the PTX-HSA-NPs suppressed MDA-MB-231 tumor growth 10-fold higher than without exposure to ultrasound. This evidence concerns the gene ALB and neoplasm.