It was previously demonstrated that the effects of other BRAF inhibitors (for example vemurafenib) can be rescued by EGFR and/or HER-3 ligands.41, 42 However, in this study, AZ304 retained its inhibitory effects on both V600E mutant and wild type BRAF CRC cell lines in the presence of the EGFR ligand EGF. Here, EGF is linked to colorectal carcinoma.