To identify the differences in TNF-induced signaling between JAK2V617F+ and JAK2V617F− cells, we cultured BM cells from Balb/c mice with established JAK2V617F-induced MPN in media containing SCF and IL-3, ±murine TNFR1 or TNFR2 BA in three independent experiments (Fig. 3a). Here, TNF is linked to myeloproliferative neoplasm.