In addition, reduction of p14ARF significantly restored the viability of Hep3B cells receiving Ad-shPRC1/taxol combined treatment (P < 0.001, Fig. 4e), suggesting that p14ARF plays a critical role in the tumor suppressor role of shPRC1 in p53-null /mutant HCC cells. This evidence concerns the gene TP53 and hepatocellular carcinoma.