HIF1A and hepatocellular carcinoma: To further elucidate that miR-3662 regulated HCC progression by targeting HIF-1α, we transfected SMMC7721-pre-miR-3662 cells with LV-HIF-1α, and HIF-1α overexpression was verified by RT-qPCR and western blotting (Fig. 6a, b).