Also highly significant was apparent preferential selection for PIK3CA H1047R compared with multiple PIK3CA mutations, including PIK3CA E545K and E542K, in breast cancer (Fig. 3b, Supplementary Fig. 9), and for NRAS Q61K and Q61R above NRAS G12D and G13D in melanoma. The gene discussed is PIK3CA; the disease is melanoma.