MUC1 and neoplasm: However, by engineering T cells to receive all 3 signals [T cell activation (signal 1) and co-stimulation (signal 2) - provided by the 2G CAR.MUC1 and cytokine support (signal 3) – provided by the 4/7ICR], we were able to achieve sustained T cell responses, highlighting the importance of recapitulating physiologic T cell signaling in a transgenic cell in order to produce durable anti-tumor effects.