We co-cultured GFP-firefly luciferase (eGFP-FFLuc) labeled MDA MB 468 breast cancer cells with either 1G or 1G.4/7ICR T cells at an effector-to-target (E:T) ratio of 1:10 in the presence of IL4 (400 U/mL), monitoring anti-tumor activity by bioluminescence imaging (Fig. 2a). This evidence concerns the gene IL4 and breast cancer.