In comparison, the percentage of remaining ChAT-positive cells in the ALS + CRD group was significantly lower than that of the ALS group at the 60-day time point (Figures 2A–C; p < 0.01) and the 90-day time point (Figures 2A–C; p < 0.05); the difference between the two groups at the 120-day time point was not significant because of the paucity of cells remaining in both the ALS + CRD and ALS groups at that time. This evidence concerns the gene CHAT and amyotrophic lateral sclerosis.