Strong positive staining was observed for markers co-localized in undifferentiated cultures by ICC (Figures 1B–D) including: GPC1 and O1 (oligodendrocyte; Figure 1B); GPC1 and CD44 (MSC marker; Figure 1C), Nestin and SDC4 (Figure 1D). The gene discussed is NES; the disease is intrahepatic cholangiocarcinoma.