In addition to measuring tau seeding in cortical gray matter regions where neuronal cell bodies and NFTs are abundant, we examined tau seeding in (1) the axons by looking at the frontal white matter tracts and optic nerve and (2) the synapses by isolating synaptosomes from different regions along the Braak Tau Pathway in an effort to determine if aggregated tau can traverse neural networks through transneuronal propagation in the human AD brain. The gene discussed is MAPT; the disease is Alzheimer disease.