As shown in Fig. 1b, 45 (53.6%) specimens from 84 patients with primary syphilis were positive for Tpp47-Tp-PCR and/or polA-Tp-PCR, including 33 specimens positive for both Tpp47-Tp-PCR and polA-Tp-PCR, 6 specimens positive only for Tpp47-Tp-PCR, and 6 specimens positive only for polA-Tp-PCR. This evidence concerns the gene POLA1 and syphilis.