Although increased levels of IL-1β and IL-6 in patients with diabetes type II have also been confirmed in different studies,57,58 down-regulation of these two pro-inflammatory genes in obesity, depression, and ND groups could be controversial because most studies have highlighted higher levels of inflammatory cytokines in all mentioned disorders.47,59,60 Thus, our results could be explained based on the cells we analyzed in this study and the protective roles of IL-1β and IL-6 in different tissues, especially CNS. Here, IL6 is linked to obesity due to melanocortin 4 receptor deficiency.