To explore the mechanism by which GRHL2 regulates TGF-β signaling and tumor development, we performed in vitro experiments using established OSCC cell lines, SCC4, SCC15, and BaP-T with stable GRHL2 knockdown through infection with various GRHL2 short hairpin RNA (shRNA) vectors targeting different sequences (pLL3.7-ShGRHL2 for SCC4 and LKO.1-ShGRHL2-1, 2, and 3 for SCC15 and BaP-T; Fig. 5a, b, Supplement Figure S2). This evidence concerns the gene GRHL2 and infection.