During infection with SeV, overexpression of OTUD1-wild type obviously lowered the activity of IFNβ promoter, whereas overexpression of OTUD1-CH mutant completely had no effect on the IFNβ promoter activity (Fig 4I), suggesting that the deubiquitinase activity of OTUD1 is important for OTUD1-mediated inhibition of IFNβ production during viral infection. This evidence concerns the gene OTUD1 and viral infectious disease.