Mothers of the cases: 24/30 (80%); 39/61 (64%) mothers of controls had laboratory-confirmed ZIKV via PRNT; 19/32 (59%) mothers of cases vs 46/62 (74%) controls reported no rash (asymptomatic).13/32 (41%) cases of neonates were either ZIKV positive by RT-PCR or IgM (serum or CSF); out of these 13 neonates with lab-confirmed ZIKV infection, 7 (53.8%) of the mothers reported rash (symptomatic).None of the neonates in control group were positive for ZIKV testing. This evidence concerns the gene CD40LG and Zika virus infectious disease.