Compared with the median methylation levels of each gene in normal breast samples, 63/67 (94%) tumor samples showed higher methylation level for AR, 53/67 (79.1%) for RASSF1, 44/69 (63.8%) for hTERT, 37/69 (53.6%) for MYC, 36/68 (52.9%) for WNT1, and 35/68 (51.5%) for ESR1. Methylation levels varied among genes with predominantly high methylation levels in hTERT, for which levels were up to 72% in tumors and 26% in normal breast samples (Figure 1A). The gene discussed is ESR1; the disease is neoplasm.