Additional immunoblotting with phospho-tau and total tau antibodies showed that tau-pS202/S205/S208 (AT8) and tau-pS262/S356 (12E8) were enriched in the sarkosyl-insoluble fraction of AD brain, in which active TAOK-pS181 was also increased (Fig. 1b). Here, MAPT is linked to Alzheimer disease.