We used 6 KPa, 10 KPa, and 16 KPa, as our previous report [16], to represent the stiffness values of normal liver tissue, fibrosis, and cirrhosis tissue. We first detected mRNA and protein expressions of LOX family members in HCC cells growing on different stiffness substrates, and found that the expressions of LOX, LOXL1, LOXL2 and LOXL4 except for LOXL3 were all significantly upregulated in MHCC97H and Hep3B cells under higher stiffness stimulation (Fig. 1a, b). The gene discussed is LOXL2; the disease is Cirrhosis.