In the study, we investigated the expression of LOX family members in HCC cells grown on different substrate stiffness and its regulation mechanism, and subsequently analyzed possible biological effects of LOXL2 on pre-metastatic niche formation such as BMDCs recruitment, fibronectin production, MMPs and CXCL12 expression,cell adhesion, etc. Here, LOXL2 is linked to hepatocellular carcinoma.