In agreement with the inhibitor-based screen, Rac1-GFP and Cdc42-GFP were transiently recruited from 10 minutes post infection (Fig 3, Rac1-GFP and Cdc42-GFP, and fluorescence intensity plots through the central plane of the EB), whereas Arf1-GFP and RhoA-GFP were not enriched at entry foci (Fig 3, Arf1-GFP and RhoA-GFP). This evidence concerns the gene ARF1 and infection.