Metformin at a dose of 300 mg/kg day, but not 100 mg/kg day, significantly reduced expression levels of both VEGF and FGF‐2 in tumours (Figure 2D‐G), indicating the involvement of VEGF and FGF‐2 signalling in metformin‐induced anti‐angiogenesis. This evidence concerns the gene FGF2 and neoplasm.