To test whether the chemokines expressed by Caco-2 cells during Giardia infection [41] can be potential substrates for CPs, we incubated 1 μg of chemokines with active mature enzymes in the presence or absence of E-64 and examined their cleavage/degradation by gel electrophoresis (Fig. 6, Table S6). This evidence concerns the gene CAD and giardiasis.