To confirm that de novo lipogenesis mediates the effect of the miR-422a–PDK2 axis on ROS production, we treated cancer cells with cerulenin, a chemical inhibitor of the key lipogenic enzyme fatty acid synthetase (FASN), or with glycerol, an compound that promotes TAG synthesis33, and assessed the rescue effects of the two substances on ROS levels. The gene discussed is FASN; the disease is cancer.