To determine the impacts of PDK2 on GC cells, we elevated PDK2 expression in MGC803 and HGC27 cells, and repressed its expression in SGC7901 and BGC823 cells by lentivirus infection and verified the altered expression of PDK2 in these GC cell lines by western blotting (Supplementary Figure 5A). This evidence concerns the gene PDK2 and gastric cancer.