In the present study, we evaluated apoptosis, viability and mitochondrial morphology of BM-derived MSCs (BM-MSCs) from the HS-27a cell line and MDS patients with IO, and explored the role of the AMPK/MFF/Drp1 pathway in MSC damage in the HS-27a cell line and MDS patients under the condition of IO. Here, PRKAA2 is linked to myelodysplastic syndrome.