For instance, RNA-seq analysis of human melanoma COLO829 cells transfected with MITF short hairpin RNA (shRNA) demonstrates an up-regulation of over 17 of the same 55 innate immune DEGs identified in Mitfmi-vga9/+ McSCs, including Ifih1, Ifit3, Irf7, Parp9, and Stat1 (GSE50686 [41]). This evidence concerns the gene IRF7 and melanoma.