Additionally, the functions of glioma‐associated oncogene 1 (GLI1) and GLI3 in liver cancer were further evaluated by depletion or activation of their expression in CD90+ cancer stem cells, and also the downstream signalling involving the interleukin‐6/Janus kinase 2 (IL6/JAK2) pathway and signal transducer and activator of transcription 3 (STAT3) phosphorylation, which might promote the application of CD90+ stem cells for liver cancer diagnosis and treatment. Here, JAK2 is linked to liver cancer.