To examine the ability of M7824 to sequester murine TGFβ and reduce TGFβ-dependent signaling, 4T1-pSMAD2-luc tumor cells, which express PD-L1 and TGFβRII and have intact TGFβ-dependent SMAD2/3 signaling (Supplementary Figure 1B, C), were used. This evidence concerns the gene TGFB1 and neoplasm.