Treatment of 4T1-pSMAD-luc tumor cells with M7824 either prior to (Fig. 1C) or after (Fig. 1D) the addition of TGFβ1 reduced TGFβ1-dependent phosphorylation of SMAD2, as indicated by decreased SMAD2 promoter-dependent luciferase activity (Fig. 1C, D). This evidence concerns the gene TGFB1 and neoplasm.