In this study, we have co-cultured the prostate cancer cell lines with a telomerase immortalized human prostate stromal myofibroblast cell line, that was either stably transduced with AR (PShTert-AR), or with empty expression vector and not expressing AR (PShTert), to determine the effect of myofibroblast AR expression on myofibroblast-prostate cancer cell interactions in vitro. Here, AR is linked to prostate cancer.