In this study, we performed the gene expression profiling of CD274 (PD-L1), GATA3, IFNG, IL12R, IL12RB2, IL4, PDCD1 (PD-1), PDCD1LG2 (PD-L2), and TBX21 (T-bet) using 158 primary GBM samples from the TCGA data set, followed by principal component analysis (PCA) to obtain Th1 and Th2 scores. The gene discussed is CD274; the disease is glioblastoma.