The purpose of this work was to establish NIR-PIT for canine invasive TCC in vitro and in vivo using the canine antibody can225IgG, which was constructed based on the exact EGFR-specific antigen-binding site of cetuximab [22] for comparative trials [23], and showed comparable affinity to the canine EGFR, which is 95% homologous to human EGFR [24]. Here, EGFR is linked to tarsal-carpal coalition syndrome.