To establish the roles of miR-449a and GPR158 in human gliomas, we first analysed 'RNA-Seq by Expectation-Maximization' (rsem) data of 431 gliomas (155 GBM and 276 low-grade gliomas (LGG), Supplementary table 3) from the TCGA database, and complemented this dataset with mRNA expression analysis and immunohistochemical detection of GPR158 protein in human gliomas from our institution (NHNN, Supplementary table 3). The gene discussed is GPR158; the disease is central nervous system cancer.