miR-449a targets CCND1 and Gpr158 are highly expressed in tumours with low miR-449a levels (i.e., in Pten/p53 and Rb/p53ant) whilst it is much less expressed in Rb/p53 (miR-449ahigh) tumours, consistent with the in vitro experiments (Fig. 5). This evidence concerns the gene RB1 and neoplasm.