These experiments showed that the anti-migratory effect associated with osteosarcoma-derived Sema3A in KHOS cells was partially reversed by the Nrp1 antibody (Figure S6), indicating the involvement of other components of Semaphorin/Nrp signaling, in particular Nrp2 and vascular endothelial growth factor (VEGF)28–30. This evidence concerns the gene NRP1 and osteosarcoma.