AKT1 and systemic sclerosis: At the same time, cultured untreated SSc fibroblasts had a rapid and significant increase in Akt phosphorylation after 15 min and 30 min (p < 0.01 vs. T0 for both time points), and ACT-333679 already contrasted this increased Akt phosphorylation after 15 min, and significantly after 30 min of treatment (p < 0.01 vs. untreated cells, for all tested concentrations) (Fig. 4b).