To test whether CLICK can be applied to other species such as rats, we designed lssDNA including two loxP sequences floxing exon 2 of the rat vesicle-associated membrane protein-associated protein B/C (Vapb) gene with a P56S mutation, which is associated with amyotrophic lateral sclerosis in humans [14] (Fig. 2b and Additional file 14: Figure S14). The gene discussed is VAPB; the disease is amyotrophic lateral sclerosis.