We analyzed the expression level of gastric cancer-related genes EGFR, FGFR2, KLF4, DNMT1 and AGO4 identified through bioinformatics screening by relative quantification method and observed differential expression of EGFR, FGFR2, KLF4, DNMT1 and AGO4 in tumors. This evidence concerns the gene DNMT1 and gastric cancer.