B3GNT8 and hepatocellular carcinoma: To explore whether β3GnT8 is required for invasion and migration of HCC cells in vitro, we knocked down the endogenous expression of β3GnT8 in SK-Hep-1, SMMC7721 and HepG2 cell lines using pSilencircle-si-β3GnT8 plasmids, which was confirmed by Western blot analysis (Figure 3A).