Furthermore, our results also showed that the levels of total polylactosamines and glycopattern abundance were significantly elevated in β3GnT8-overexpressing HCC cells, but were markedly reduced in β3GnT8-deficient cells, consistent with previous findings that polylactosamines are synthesized by β3GnT family in which β3GnT8 is a key member [9]. This evidence concerns the gene B3GNT2 and hepatocellular carcinoma.