Fifth, the translational relevance of 2HF was further confirmed by its characteristic down-regulation of critical MammaPrint genes including CCNE2, LPCAT1, MCM6, DCK, GNAZ, ECI2, DTL, MELK, NUSAP, NMU, 1and EGLN1 in MCF7, SKBR3 and MDA-MB-231 BC cells. This evidence concerns the gene ECI2 and breast cancer.