TNF and rheumatoid arthritis: In such regard, RNA-Seq experiments made by Tamarozzi et al. (13), using neutrophils isolated by negative-selection (>99.9% pure) from HDs or RA patients (as we do), then treated for 1 h with a range of inflammatory cytokines (TNFα, GM-CSF, G-CSF, IL-6, IL-1β, CXCL8, IFNα, and IFNγ), also failed to detect any of the mRNA for IL-17 cytokine family.