To verify bradyzoite-specific proteins are expressed in replicating tachyzoites lacking AP2IV-4, we completed immunofluorescence assays (IFA) of PruQ-Δap2IV-4 (Fig 3B and 3C) as well as RHCre-Δap2IV-4 tachyzoites (S4 Fig) using antibodies to four bradyzoite specific proteins (α-BPK1, α-MCP4, α-CST1, α-SRS9) and also evaluated the formation of cyst walls using biotin-labeled Dolichos biflorus agglutinin (DBA). The gene discussed is RPS19; the disease is cyst.